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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in several tumors, which results in chromosome instability, and thus may perhaps contribute to tumorigenesis. This protein was uncovered to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a essential part in promoting mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants ended up noted but detailed nucleotide sequence data isn't nonetheless offered.
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Blocking peptides are peptides that bind specifically for the goal antibody and block antibody binding. These peptide ordinarily consists of the epitope identified by the antibody.
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This guidebook outlines additional possible optimization of cellular and immunoassay parameters to be certain the absolute best final results are...
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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies sure to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining with check here the blocked antibody as opposed to the antibody on your own, one can see which staining is particular.
Target Information Cyclin E belongs into the really conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which lead towards the temporal coordination of each mitotic occasion. Cyclin E sorts a fancy with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S stage boundary and is degraded as cells development by means of S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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